Lab

Determining the Affect of Subst mark Concentration on the stain Rate of Reaction for a Catalase Extract (from Beef Liver) in a 3 Minute Period Introduction: In this investigation, it go away be determined whether the changing subst enjoy preoccupation (through dilution of 3% hydrogen hydrogen peroxide) has an effect on the tramp reception with catalase evoke (of beef liver). The substratum that bequeath be utilize is hydrogen peroxide (H2O2). An enzyme which accelerates the breakdown of hydrogen peroxide into water and atomic number 8 gas is know as a catalase. 2H2O2--------catalase--------------> 2H2O + O2 A agency which decreases the activation vim of a chemical answer and ontogeny the rate of response is a catalyst. So a catalase is an organic fertilizer catalyst. In this experiment, hydrogen peroxide will be used as the substrate for the catalase. Enzyme activity is proportionally to substrate soaking up at lo w substrate concentration. At higher substrate concentration there are more collisions involving the substrate and active site. Since the active sites are all taken up in high substrate concentration for the enzyme, when the substrate concentration is improver there is no effect. The graph below shows the change in the rate of answer with higher substrate concentration, where the rate of reaction reaches a peak then is leveled off: Source: hypertext transplant protocol://www.rsc.org/education/teachers/learnnet/cfb/enzymes.htm In this experiment, the substrate concentration will be manipulated by diluting the 3% hydrogen peroxide. Furthermore, the initial reaction rate with the catalase extract (beef liver) will be determined by the atomic number 8 output in intervals of 20 seconds for a period of 3 minutes (180 seconds). Research scruple: What is the affect of the change in substrate concentration (dilution of 3% hydrogen peroxide) on the rate of rate wit h catalase extract (beef liver)? specul! ation: As the substrate concentration (of...If you want to get a full essay, order it on our website: BestEssayCheap.com

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